SrasV1, Main, Exploration, bibRecord, 002A12

Simultaneous detection of five biothreat agents in powder samples by a multiplexed suspension array.

Identifieur interne : 002A12 ( Main/Exploration ); précédent : 002A11; suivant : 002A13

Simultaneous detection of five biothreat agents in powder samples by a multiplexed suspension array.

Auteurs : Jing Wang [République populaire de Chine] ; Yu Yang ; Lei Zhou ; Jinglin Wang ; Yongqiang Jiang ; Kongxin Hu ; Xiaohong Sun ; Yousong Hou ; Ziwen Zhu ; Zhaobiao Guo ; Yanli Ding ; Ruifu Yang

Source :

Immunopharmacology and immunotoxicology [ 1532-2513 ] ; 2009.

RBID : pubmed:19555207

Descripteurs français

English descriptors

KwdEn :
- Bacillus anthracis, Biological Warfare Agents, Enterotoxins, Immunoassay (methods), Microarray Analysis (methods), Ricin, SARS Virus, Sensitivity and Specificity, Spores, Bacterial, Yersinia pestis.
MESH :
- chemical : Biological Warfare Agents, Enterotoxins, Ricin.
- methods : Immunoassay, Microarray Analysis.
- Bacillus anthracis, SARS Virus, Sensitivity and Specificity, Spores, Bacterial, Yersinia pestis.

Abstract

A suspension array-based multiplexed immunoassay was developed for rapid, sensitive, specific, and simultaneous detection of multiple biothreat-associated agents in powder samples. The 5-plexed immunoassays using sets of 9-plexed coupled fluorescent beads were employed to simultaneously detect five representative biothreat agents, including B. anthracis spore, Y. pestis, SARS-CoV, staphylococcal enterotoxin B (SEB) and ricin from a single powder sample and the feasibility for field samples was demonstrated by both blinded and standard laboratory trials. The detection sensitivity and dynamic range for the five biothreat agents from different powders might be varied depending on the nature of the powder and the feature of the contaminating agent. The limit of detection for Y. pestis, B. anthracis spores, SEB, ricin, SARS-CoV N protein in milk powder was 20 cfu, 111 cfu, 110pg, 5.4 ng and 2 ng per test respectively. Compared to conventional ELISA method, the suspension array has a higher sensitive ability, and can detect five biothreat agents simultaneously with high reproducibility.

DOI: 10.1080/08923970902740837
PubMed: 19555207

Affiliations:

Le document en format XML

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<front><div type="abstract" xml:lang="en">A suspension array-based multiplexed immunoassay was developed for rapid, sensitive, specific, and simultaneous detection of multiple biothreat-associated agents in powder samples. The 5-plexed immunoassays using sets of 9-plexed coupled fluorescent beads were employed to simultaneously detect five representative biothreat agents, including B. anthracis spore, Y. pestis, SARS-CoV, staphylococcal enterotoxin B (SEB) and ricin from a single powder sample and the feasibility for field samples was demonstrated by both blinded and standard laboratory trials. The detection sensitivity and dynamic range for the five biothreat agents from different powders might be varied depending on the nature of the powder and the feature of the contaminating agent. The limit of detection for Y. pestis, B. anthracis spores, SEB, ricin, SARS-CoV N protein in milk powder was 20 cfu, 111 cfu, 110pg, 5.4 ng and 2 ng per test respectively. Compared to conventional ELISA method, the suspension array has a higher sensitive ability, and can detect five biothreat agents simultaneously with high reproducibility.</div>
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<name sortKey="Hou, Yousong" sort="Hou, Yousong" uniqKey="Hou Y" first="Yousong" last="Hou">Yousong Hou</name>
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Simultaneous detection of five biothreat agents in powder samples by a multiplexed suspension array.

Simultaneous detection of five biothreat agents in powder samples by a multiplexed suspension array.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki

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